Influence of Soaking Duration and Temperature on the Physicochemical and Functional Properties of a Tunisian Chickpea Cultivar

Background: Chickpea (Cicer arietinum L.) is a widely consumed legume renowned for its high nutritional value. However, its utilization is constrained by the presence of antinutritional factors, such as phytates and tannins, which may compromise nutrient bioavailability. Thermal processing, particularly soaking conditions, has been reported as an effective approach for enhancing nutritional quality and reducing antinutritional compounds.

Aims: This study aimed to evaluate the effect of thermal parameters—specifically soaking time and temperature—on the nutritional and antinutritional quality of chickpeas, and to optimize these conditions utilizing a statistical experimental design.

Patients and Methods: A Central Composite Design (CCD) was implemented employing Design-Expert software to assess the combined effects of soaking temperature (20 °C, 40 °C, and 60 °C) and soaking time (6 h, 15 h, and 24 h). Chickpea samples were analyzed for physicochemical properties (dry matter, pH, and conductivity), nutritional components (crude protein and fiber contents), antinutritional factors (phytates and condensed tannins), and functional properties, including swelling and hydration capacities.

Results: Physicochemical parameters remained relatively stable across all thermal treatments. In contrast, soaking at 20 °C for 24 h resulted in increased protein and fiber contents, accompanied by a significant reduction in antinutritional factors, with decreases of 29.68% in phytates and 33.42% in condensed tannin content. Furthermore, functional properties, including swelling and hydration capacities were significantly improved under these optimized conditions.

Conclusions: Optimization of soaking time and temperature constitutes an effective strategy for enhancing the nutritional and functional properties of chickpeas while substantially reducing antinutritional factors. These findings underscore the potential of controlled thermal treatments to improve chickpea quality for food applications.

Keywords

Chickpea Thermal Treatment Soaking Parameters Nutritional Quality Antinutritional Factors
  • Sarra Sammoud
    Laboratory of Innovation and Valorization for Sustainable Food Industry Higher School of Food Industries of Tunis, University of Carthage, 58 Avenue Alain Savary, 1003, Tunis, Tunisia
  • Ichrak Charfi
    Bouebdelli University (BU) 30 Avenue Kheireddine Pacha, 1002, Tunis, Tunisia
  • Mariem Bannour
    Bouebdelli University (BU), 30 Avenue Kheireddine Pacha, 1002, Tunis, Tunisia
  • Safa Barakati
    Laboratoire des Substances Naturelles, Institut National de Recherche et d’Analyses Physico-chimiques (INRAP) BiotechPole Sidi Thabet, 2020. Institut National Agronomique de Tunis (INAT) 43 Av. Charles Nicolle, Tunis 1082, Tunisia
  • Salwa Bornaz
    Laboratory of Innovation and Valorization for Sustainable Food Industry Higher School of Food Industries of Tunis, University of Carthage, 58 Avenue Alain Savary, 1003, Tunis, Tunisia

How to Cite

Sammoud, S., Charfi, I., Bannour, M., Barakati, S., & Bornaz, S. (2026). Influence of Soaking Duration and Temperature on the Physicochemical and Functional Properties of a Tunisian Chickpea Cultivar. The North African Journal of Food and Nutrition Research, 10(21), 192-204. https://doi.org/10.51745/najfnr.10.21.192-204

Agume, A. S. N., Njintang, N. Y., & Mbofung, C. M. F. (2017). Effect of soaking and roasting on the physicochemical and pasting properties of soybean flour. Foods, 6(2), Article 12. https://doi.org/10.3390/foods6020012 DOI: https://doi.org/10.3390/foods6020012

Amri, M., Kharrat, M., & Ben Salah, H. (2016). La variété de pois chiche « REBHA ». Annales de l’INRAT – Spécial Innovations, 17–19. https://doi.org/10.12816/0028684 DOI: https://doi.org/10.12816/0028684

AOAC. (1995). Official methods of analysis (16th ed.). Association of Official Analytical Chemists. https://www.cabidigitallibrary.org/doi/full/10.5555/19951414840

Aubert, G. (1978). Méthodes d'analyses du sol (2e éd.). C.N.D.P.

Bayram, M., Kaya, A., & Öner, M. D. (2004). Changes in properties of soaking water during production of soy-bulgur. Journal of Food Engineering, 61(2), 221–230. https://doi.org/10.1016/s0260-8774(03)00094-3 DOI: https://doi.org/10.1016/S0260-8774(03)00094-3

Boukid, F. (2021). Chickpea (Cicer arietinum L.) protein as a prospective plant‐based ingredient: A review. International Journal of Food Science & Technology, 56(11), 5435–5444. https://doi.org/10.1111/ijfs.15046 DOI: https://doi.org/10.1111/ijfs.15046

Boye, J., Zare, F., & Pletch, A. (2010). Pulse proteins: Processing, characterization, functional properties and applications in food and feed. Food Research International, 43(2), 414–431. https://doi.org/10.1016/j.foodres.2009.09.003 DOI: https://doi.org/10.1016/j.foodres.2009.09.003

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3 DOI: https://doi.org/10.1016/0003-2697(76)90527-3

Cid-Gallegos, M. S., Corzo-Ríos, L. J., Jiménez-Martínez, C., & Sánchez-Chino, X. M. (2022). Protease inhibitors from plants as therapeutic agents- a review. Plant Foods for Human Nutrition. https://doi.org/10.1007/s11130-022-00949-4 DOI: https://doi.org/10.1007/s11130-022-00949-4

Coffigniez, F., Briffaz, A., Mestres, C., Akissoé, L., Bohuon, P., & El Maâtaoui, M. (2019). Impact of soaking process on the microstructure of cowpea seeds in relation to solid losses and water absorption. Food Research International, 119, 268–275. https://doi.org/10.1016/j.foodres.2018.10.069 DOI: https://doi.org/10.1016/j.foodres.2019.02.010

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