Inactivation of E. faecalis under microwave heat treatment and ultrasound probe

Nouara Kernou (1) , Amine Belbahi (2) , Kenza Bedjaoui (3) , Ghania Kaanin-Boudraa (4) , Lila Boulekbache-Makhlouf (5) , Khodir Madani (6)
(1) Laboratoire Biomathématiques Biophysique Biochimie et de Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia , Algeria
(2) Department of Microbiology and Biochemistry, Faculty of Sciences, University of M’Sila, M’Sila , Algeria
(3) Laboratoire Biomathématiques Biophysique Biochimie et de Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia , Algeria
(4) Laboratoire Biomathématiques Biophysique Biochimie et de Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia , Algeria
(5) Laboratoire Biomathématiques Biophysique Biochimie et de Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia , Algeria
(6) Centre de Recherche en Technologies Agro-alimentaires (CRTAA), Campus universitaire Targua Ouzemour. Bejaia. 06000 , Algeria

Abstract

Background and aims: The Weibull model was fitted to survival curves in order to describe inactivation kinetics, and the effect of combined microwave (MW) and ultrasound (US) treatments was evaluated. Methods: Enterococcus faecalis ATCC 29212 present in 40 mL of sterile physiological water was treated with microwaves at 300W, 600W, and 900W and/or ultrasonic probes (amplitude 60 %, 80 % and 100 %, pulse (3s continuous, 3s discontinuous). Results: The use of an ultrasonic probe at 20 kHz displayed no significant impact on the patients' ability to survive. At 600 W and 300 W of MW treatment, a decrease of 3.96 log and 0.90 log, respectively, was obtained. Total destruction was accomplished in 70 seconds when 900 W of microwave therapy was used. Additionally, it was shown that the effectiveness of WM and US increased with increasing power and exposure duration. This was the case even when microwave or ultrasonic technology was utilized independently. In addition, the treatment that included both microwaves and ultrasound showed a significantly better effect than the treatment that only involved microwaves, but there were no significant differences between the coupled treatment and the microwave treatment given for 30 seconds. Conclusions: The results of the current study show that the inactivation of Enterococcus faecalis by ultrasound followed by microwave treatment was significantly higher than that obtained by microwave treatment followed by ultrasound.

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Authors

Nouara Kernou
nouara89@gmail.com (Primary Contact)
Amine Belbahi
Kenza Bedjaoui
Ghania Kaanin-Boudraa
Lila Boulekbache-Makhlouf
Khodir Madani
Kernou, N., Belbahi, A., Bedjaoui, K., Kaanin-Boudraa , G. ., Boulekbache-Makhlouf, L., & Madani, K. (2023). Inactivation of E. faecalis under microwave heat treatment and ultrasound probe. The North African Journal of Food and Nutrition Research, 7(15), 52–58. https://doi.org/10.51745/najfnr.7.15.52-58

Article Details

Received 2023-01-10
Accepted 2023-03-12
Published 2023-03-20

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