A UV-Visible spectrophotometric approach to quantify caffeine and carbohydrates in Dhaka's chocolate and candy selection, Bangladesh
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Abstract
Background: Caffeine and carbohydrates are significant components in chocolate and confectionery products, influencing both their sensory appeal and health impact. Accurate quantification of these components is essential to provide consumers with reliable nutritional information and to inform healthier dietary choices.
Aims: This study aims to quantify the moisture and ash content, as well as determine the concentrations of caffeine and carbohydrates, using UV-Visible spectrophotometry, in selections of chocolates and candies commercially available in Dhaka, Bangladesh. Additionally, the study seeks to elucidate variations in these components across different brands and product types, thereby contributing to a better understanding of their compositional profiles.
Methods: Samples from six chocolate brands and two coffee candy brands were procured from local markets and supermarkets in Dhaka. Standard analytical methodologies were employed to determine moisture and ash content. For the quantification of caffeine and carbohydrates, standard solutions were prepared, and absorbance measurements were conducted using a UV-Visible spectrophotometer at specified wavelengths. Moisture and ash content were calculated using standard formulas, while caffeine and carbohydrate concentrations were derived from calibration curves.
Results: The moisture content in the analyzed samples ranged from 0.25% to 1.88%, while ash content varied between 5.22% and 6.90%. Caffeine concentrations were found to range from 24.18 ± 2.51 mg.kg-1 in Perk chocolate to 60.12 ± 0.85 mg.kg-1 in KitKat (70% Dark). Carbohydrate content exhibited considerable variation, with values ranging from 2.03 ± 0.01 g/100 g in Coffee Bite to 41.05 ± 0.46 g/100 g in Perk chocolate per 100 g. Dark chocolate samples demonstrated higher caffeine levels compared to milk and white chocolate varieties, consistent with their elevated cocoa solid content.
Conclusions: The study revealed significant variability in moisture, ash, caffeine, and carbohydrate content across different chocolate and candy brands. These findings emphasize the necessity of rigorous compositional analysis for quality assurance and public health considerations.
Keywords: Caffeine, Carbohydrates, Chocolate, Candy, UV-Visible Spectrophotometry.
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References
Addicott, M. A., Yang, L. L., Peiffer, A. M., Burnett, L. R., Burdette, J. H., Chen, M. Y., Hayasaka, S., Kraft, R. A., Maldjian, J. A., & Laurienti, P. J. (2009). The effect of daily caffeine use on cerebral blood flow: How much caffeine can we tolerate? Human Brain Mapping, 30(10), 3102–3114. https://doi.org/10.1002/hbm.20732
Afoakwa, E. O., Paterson, A., Fowler, M., & Ryan, A. (2008). Flavor Formation and Character in Cocoa and Chocolate: A Critical review. Critical Reviews in Food Science and Nutrition, 48(9), 840–857. https://doi.org/10.1080/10408390701719272
Ahn, J. Y., Kil, D. Y., Kong, C., & Kim, B. G. (2014). Comparison of Oven-drying Methods for Determination of Moisture Content in Feed Ingredients. Asian-Australasian Journal of Animal Sciences, 27(11), 1615–1622. https://doi.org/10.5713/ajas.2014.14305
Al-Bratty, M., Alhazmi, H. A., Rehman, Z. U., Javed, S. A., Ahsan, W., Najmi, A., Khuwaja, G., Makeen, H. A., & Khalid, A. (2020). Determination of caffeine content in commercial energy beverages available in Saudi Arabian market by gas Chromatography-Mass spectrometric analysis. Journal of Spectroscopy, 2020, 1–9. https://doi.org/10.1155/2020/3716343
AOAC Official Method 2001.12Water/Dry matter (Moisture) in animal feed, grain, and forage (Plant tissue). (2023). In Oxford University Press eBooks. https://doi.org/10.1093/9780197610145.003.1388
Ashihara, H., Mizuno, K., Yokota, T., & Crozier, A. (2017). Xanthine Alkaloids: Occurrence, Biosynthesis, and Function in Plants. Progress in the chemistry of organic natural products, 105, 1–88. https://doi.org/10.1007/978-3-319-49712-9_1
AZoM. (2014). Moisture and fat analysis in chocolate. Retrieved 9 June 2024 from https://www.azom.com/article.aspx?ArticleID=11472
Belitz, H.-D., Grosch, W., & Schieberle, P. (2009). Food Chemistry (4th ed.). Springer. https://doi.org/10.1007/978-3-540-69934-7
Bhandari, B., Bansal, N., Zhang, M., & Schuck, P. (2013). Handbook of Food Powders: Processes and Properties. Woodhead Publishing. http://dx.doi.org/10.1533/9780857098672
Del Río-Celestino, M., & Font, R. (2020). The Health Benefits of Fruits and Vegetables. Foods (Basel, Switzerland), 9(3), 369. https://doi.org/10.3390/foods9030369
EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA). (2015). Scientific Opinion on the safety of caffeine. EFSA Journal, 13(5). https://doi.org/10.2903/j.efsa.2015.4102
Ergun, R., Lietha, R., & Hartel, R. W. (2010). Moisture and shelf life in sugar confections. Critical Reviews in Food Science and Nutrition, 50(2), 162–192. https://doi.org/10.1080/10408390802248833
Geraghty, S. R., McNamara, K., Kwiek, J. J., Rogers, L., Klebanoff, M. A., Augustine, M., & Keim, S. A. (2015). Tobacco Metabolites and Caffeine in Human Milk Purchased via the Internet. Breastfeeding Medicine: The Official Journal of the Academy of Breastfeeding Medicine, 10(9), 419–424. https://doi.org/10.1089/bfm.2015.0096
Habtamu, D., & Belay, A. (2020). First order derivative spectra to determine caffeine and chlorogenic acids in defective and non-defective coffee beans. Food Science & Nutrition, 8(9), 4757–4762. https://doi.org/10.1002/fsn3.1723
Harris, G. K., & Marshall, M. R. (2017). Ash Analysis. In Food Science Text Series (pp. 287–297). Springer International Publishing. https://doi.org/10.1007/978-3-319-45776-5_16
Institute of Medicine (US) Committee on Military Nutrition Research. (2001). Caffeine for the Sustainment of Mental Task Performance: Formulations for Military Operations. National Academies Press (US). https://doi.org/10.17226/10219
Ismail, B. P. (2017). Ash Content Determination. In Food Analysis Laboratory Manual (pp. 117–119). Springer International Publishing. https://doi.org/10.1007/978-3-319-44127-6_11
Jackson, E.B. (1995). Sugar confectionery manufacture. (2nd ed.). Springer.
Jahrami, H., Al-Mutarid, M., Penson, P. E., Al-Islam Faris, M., Saif, Z., & Hammad, L. (2020). Intake of Caffeine and Its Association with Physical and Mental Health Status among University Students in Bahrain. Foods (Basel, Switzerland), 9(4), 473. https://doi.org/10.3390/foods9040473
Jeon, J. S., Kim, H. T., Jeong, I. H., Hong, S. R., Oh, M. S., Yoon, M. H., Shim, J. H., Jeong, J. H., & Abd El-Aty, A. M. (2019). Contents of chlorogenic acids and caffeine in various coffee-related products. Journal of Advanced Research, 17, 85–94. https://doi.org/10.1016/j.jare.2019.01.002
Katz, D. L., Doughty, K., & Ali, A. (2011). Cocoa and chocolate in human health and disease. Antioxidants & Redox Signaling, 15(10), 2779–2811. https://doi.org/10.1089/ars.2010.3697
Khowala, S., Verma, D., & Banik, S. P. (2008). Biomolecules: (Introduction, Structure and Function): Carbohydrates. National Science Digital Library, 6, 1-93.
Kirk, R. S., Sawyer, R., & Egan, H. (Eds.). (1991). Pearson’s Composition and Analysis of Foods (9th ed.). Longman. http://ci.nii.ac.jp/ncid/BA13386742
Kusumadevi, Z., Saputro, A. D., Dewi, A. K., Irmandharu, F., Oetama, T., Setiowati, A. D., Rahayoe, S., & Bintoro, N. (2021). Physical characteristics of compound chocolate made with various flavouring agents produced using melanger as a small-scale chocolate processing device. IOP Conference Series. Earth and Environmental Science, 653(1), 012036. https://doi.org/10.1088/1755-1315/653/1/012036
Lisko, J. G., Lee, G. E., Kimbrell, J. B., Rybak, M. E., Valentin-Blasini, L., & Watson, C. H. (2017). Caffeine Concentrations in Coffee, Tea, Chocolate, and Energy Drink Flavored E-liquids. Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco, 19(4), 484–492. https://doi.org/10.1093/ntr/ntw192
Ludwig, I. A., Mena, P., Calani, L., Cid, C., Del Rio, D., Lean, M. E., & Crozier, A. (2014). Variations in caffeine and chlorogenic acid contents of coffees: what are we drinking? Food & function, 5(8), 1718–1726. https://doi.org/10.1039/c4fo00290c
Menezes, E. W., de Melo, A. T., Lima, G. H., &Lajolo, F. M. (2004). Measurement of carbohydrate components and their impact on energy value of foods. Journal of Food Composition and Analysis, 17(3-4), 331–338. https://doi.org/10.1016/j.jfca.2004.03.018
Minifie, B. W. (1989). Chocolate, cocoa, and confectionery: Science and Technology (3rd ed.). Van Nostrand Reinhold. https://doi.org/10.1007/978-94-011-7924-9
Müller, C., Vetter, F., Richter, E., & Bracher, F. (2014). Determination of caffeine, myosmine, and nicotine in chocolate by headspace solid-phase microextraction coupled with gas chromatography-tandem mass spectrometry. Journal of Food Science, 79(2), T251–T255. https://doi.org/10.1111/1750-3841.12339
Nafingah, R., Kurniasari, J., Cahyani, A., Harmayani, E., & Saputro, A. D. (2019). Investigating the impact of Palm Sap Sugar proportion and fat content on heat stability of Milk Chocolate. IOP Conference Series. Earth and Environmental Science, 355(1), 012043. https://doi.org/10.1088/1755-1315/355/1/012043
Nielsen, S. S. (2017). Moisture content determination. In Food science text series, 105–115. https://doi.org/10.1007/978-3-319-44127-6_10
Observatory of Economic Complexity (OEC) Chocolate in Bangladesh. (2022). The Observatory of Economic Complexity. Retrieved June 10, 2024, from https://oec.world/en/profile/bilateral-product/chocolate/reporter/bgd
Ouhakki H, Elfallah K, Adiba A, Hamid T, Elmejdoub N. (2024). Investigation of the water quality in Oum Er Rbia River (Morocco): A multifaceted analysis of physicochemical, undesirable substances, toxic compounds, and bacteriological traits. Tropical Journal of Natural Product Research, 8(4). https://doi.org/10.26538/tjnpr/v8i4.11
Pomeranz, Y., & Meloan, C. E. (1994). Ash and Minerals. In Food Analysis, (pp. 602–624). Springer US. https://doi.org/10.1007/978-1-4615-6998-5_35
Roehrs, T., & Roth, T. (2008). Caffeine: sleep and daytime sleepiness. Sleep Medicine Reviews, 12(2), 153–162. https://doi.org/10.1016/j.smrv.2007.07.004
Saputro, A. D., Van De Walle, D., Aidoo, R. P., Mensah, M. A., Delbaere, C., De Clercq, N., Van Durme, J., & Dewettinck, K. (2017). Quality attributes of dark chocolates formulated with palm sap-based sugar as nutritious and natural alternative sweetener. European Food Research & Technology, 243(2), 177–191. https://doi.org/10.1007/s00217-016-2734-9
Saraiva, S. M., Jacinto, T. A., Gonçalves, A. C., Gaspar, D., & Silva, L. R. (2023). Overview of Caffeine Effects on Human Health and Emerging Delivery Strategies. Pharmaceuticals (Basel, Switzerland), 16(8), 1067. https://doi.org/10.3390/ph16081067
Sereshti, H., & Samadi, S. (2014). A rapid and simple determination of caffeine in teas, coffees and eight beverages. Food chemistry, 158, 8–13. https://doi.org/10.1016/j.foodchem.2014.02.095
Sultana, A., Haque, M. S., Shoeb, M., Islam, M. S., Mamun, M. I. R., & Nahar, N. (2012). Presence of yellow 6, an artificial colour additive in orange juice. Journal of the Bangladesh Chemical Society, 25(1), 80–86. https://doi.org/10.3329/jbcs.v25i1.11778
Sun, S., Xie, Y., Yang, R., Zhu, M., Sablani, S., & Tang, J. (2023). The influence of temperature and water activity on thermal resistance of Salmonella in milk chocolate. Food Control, 143, 109292. https://doi.org/10.1016/j.foodcont.2022.109292
Temple, J. L., Bernard, C., Lipshultz, S. E., Czachor, J. D., Westphal, J. A., & Mestre, M. A. (2017). The Safety of Ingested Caffeine: A Comprehensive Review. Frontiers in psychiatry, 8, 80. https://doi.org/10.3389/fpsyt.2017.00080
Vieira, A. J., Gaspar, E. M., & Santos, P. M. (2020). Mechanisms of potential antioxidant activity of caffeine. Radiation Physics and Chemistry, 174, 108968. https://doi.org/10.1016/j.radphyschem.2020.108968
Weibel, J., Lin, Y. S., Landolt, H. P., Berthomier, C., Brandewinder, M., Kistler, J., Rehm, S., Rentsch, K. M., Meyer, M., Borgwardt, S., Cajochen, C., & Reichert, C. F. (2021). Regular Caffeine Intake Delays REM Sleep Promotion and Attenuates Sleep Quality in Healthy Men. Journal of Biological Rhythms, 36(4), 384–394. https://doi.org/10.1177/07487304211013995
Zhao, F., Lin, H. T., Zhang, S., Lin, Y. F., Yang, J. F., & Ye, N. X. (2014). Simultaneous determination of caffeine and some selected polyphenols in Wuyi Rock tea by high-performance liquid chromatography. Journal of Agricultural and Food Chemistry, 62(13), 2772–2781. https://doi.org/10.1021/jf4056314
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