Nutritional Optimization and Sensory Characterization of Cookies Via Pearl Millet (Pennisetum glaucum) Flour Substitution: A Multivariate Analytical Approach
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Pearl Millet Floor, Nutritional Fortification, Proximate Composition, Sensory Evaluation, Principal Component Analysis (PCA)
Abstract
Background: Given the escalating prevalence of malnutrition and diet-related non-communicable diseases, there is an urgent demand for nutritious, affordable, and sustainable food vehicles. The integration of underutilized, climate-resilient cereals—such as pearl millet—into widely consumed bakery products represents a strategic intervention to address food security and improve public health nutrition.
Objectives: This study aimed to evaluate the impact of substituting wheat flour with graded levels of pearl millet flour (PMF) on the physicochemical composition and sensory attributes of functional cookies.
Methods: Pearl millet flour was incorporated as a partial-to-total replacement for wheat flour at levels of 0% (control), 20%, 40%, 60%, 80%, and 100%. The resulting formulations were analyzed for nutritional composition using AOAC standardized protocols and for sensory attributes employing a nine-point hedonic scale. Statistical significance was determined via ANOVA and post-hoc mean separation tests (p < 0.05), while Principal Component Analysis (PCA) was applied to characterize multivariate nutritional patterns across the experimental groups.
Results: Increasing PMF inclusion led to significant linear improvements in crude protein, ash, dietary fiber, and essential mineral content (p < 0.05). Specifically, the 40% PMF substitution exhibited a significant nutritional fortification compared to the control without compromising overall sensory acceptability. While sensory evaluation confirmed that formulations containing 20–40% PMF were acceptable comparable to the wheat-based control, higher substitution levels (≥ 60%) resulted in significantly lower scores for texture and visual appearance. Multivariate PCA demonstrated a distinct clustering of nutrient-dense profiles associated with increasing PMF levels, confirming a coordinated enhancement of the mineral and protein matrices.
Conclusion: The partial substitution of wheat flour with 20–40% pearl millet flour offers a viable approach for developing nutritionally superior cookies with high consumer acceptance. These findings support the broader application of PMF in the commercial bakery sector, school feeding programs, and therapeutic food formulation, contributing to both dietary diversity and agricultural sustainability.
Keywords: Pearl Millet Floor; Nutritional Fortification; Proximate Composition; Sensory Evaluation; Principal Component Analysis (PCA).
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References
AOAC International. (2016). Official methods of analysis of AOAC International (20th ed.). AOAC International.
Bala, A., Gul, K., & Riar, C. S. (2014). Functional and sensory properties of cookies prepared from wheat flour supplemented with cassava and water chestnut flours. Cogent Food & Agriculture, 1(1), 1–10. https://doi.org/10.1080/23311932.2014.945919
Bheemaiah Balyatanda, S., Gowda, N. A. N., Subbiah, J., Chakraborty, S., Prasad, P. V. V., & Siliveru, K. (2024). Physiochemical, bio, thermal, and non-thermal processing of major and minor millets: A comprehensive review on antinutritional and antioxidant properties. Foods (Basel, Switzerland), 13(22), 3684. https://doi.org/10.3390/foods13223684
Białek-Dratwa, A., Szczepańska, E., Szymańska, D., Grajek, M., Krupa-Kotara, K., & Kowalski, O. (2022). Neophobia—A natural developmental stage or feeding difficulties for children? Nutrients, 14(7), 1521. https://doi.org/10.3390/nu14071521
Chandrasekara, A., & Shahidi, F. (2011). Antioxidative potential of millet grains and their fractions. Journal of Agricultural and Food Chemistry, 59(17), 9566–9571. https://doi.org/10.1021/jf201849d
Cheng, A., & Bhat, R. (2016). Functional, physicochemical and sensory properties of novel cookies produced by utilizing underutilized jering (Pithecellobium jiringa) legume flour. Food Bioscience, 14, 54–61. https://doi.org/10.1016/j.fbio.2016.03.002
Devi, P. B., Vijayabharathi, R., Sathyabama, S., Malleshi, N. G., & Priyadarisini, V. B. (2014). Health benefits of finger millet (Eleusine coracana L.) polyphenols and dietary fiber: A review. Journal of Food Science and Technology, 51(6), 1021–1040. https://doi.org/10.1007/s13197-011-0584-9
FAO, IFAD, UNICEF, WFP, & WHO. (2022). The state of food security and nutrition in the world 2022. FAO. https://doi.org/10.4060/cc0639en
Grand View Research. (2023). Cookies market size, share & trends analysis report by product (bar, sandwich, drop, molded), by distribution channel (offline, online), by region, and segment forecasts, 2023–2030.
Kaur, G., Ahmadzadeh-Hashemi, S., Amir, S., et al. (2024). Exploring sustainable novel millet protein: A look at the future foods through innovative processing. Future Foods, 9, 100315.
Kaur, M., Sandhu, K. S., Arora, A., & Sharma, A. (2019). Gluten-free biscuits prepared from buckwheat flour by incorporation of various gums: Physicochemical and sensory properties. LWT – Food Science and Technology, 62(1), 628–632.
Kiani, A. K., Dhuli, K., Donato, K., et al. (2022). Main nutritional deficiencies. Journal of Preventive Medicine and Hygiene, 63(2 Suppl 3), E93–E101. https://doi.org/10.15167/2421-4248/jpmh2022.63.2S3.2752
Meena, K. K., Meena, S., Joshi, M., & Dhotre, A. V. (2024). Nutritional and functional exploration of pearl millet and its processing and utilization: An overview. Food and Humanity, 3, 100334. https://doi.org/10.1016/j.foohum.2024.100334
Navinraj, S., Rajagopalan, V. R., Swaminathan, M., Doraiswamy, U., Karthikeyan, S., Manickam, S., & Raveendran, M. (2025). Unveiling the antinutritional factors in millets: A comprehensive review. ACS Food Science & Technology, 5(6), 2046–2063. https://doi.org/10.1021/acsfoodscitech.5c00115
Obilana, A. B., & Manyasa, E. (2002). Millets. In P. S. Belton & J. R. N. Taylor (Eds.), Pseudocereals and less common cereals: Grain properties and utilization potential (pp. 177–217). Springer.
Pareyt, B., & Delcour, J. A. (2008). The role of wheat flour constituents, sugar, and fat in low-moisture cereal-based products: A review on sugar-snap cookies. Critical Reviews in Food Science and Nutrition, 48(9), 824–839. https://doi.org/10.1080/10408390701719223
Pliner, P., & Hobden, K. (1992). Development of a scale to measure the trait of food neophobia in humans. Appetite, 19(2), 105–120. https://doi.org/10.1016/0195-6663(92)90014-W
Priya, Verma, R. K., Lakhawat, S., et al. (2023). Millets: Sustainable treasure house of bioactive components. International Journal of Food Properties, 26(1), 1822–1840. https://doi.org/10.1080/10942912.2023.2236317
Rai, K. N., Gowda, C. L. L., Reddy, B. V. S., & Sehgal, S. (2008). Adaptation and potential uses of sorghum and pearl millet in alternative and health foods. Comprehensive Reviews in Food Science and Food Safety, 7(4), 340–352. https://doi.org/10.1111/j.1541-4337.2008.00049.x
Saleh, A. S. M., Zhang, Q., Chen, J., & Shen, Q. (2013). Millet grains: Nutritional quality, processing, and potential health benefits. Comprehensive Reviews in Food Science and Food Safety, 12(3), 281–295. https://doi.org/10.1111/1541-4337.12012
Satyavathi, C. T., Ambawat, S., Khandelwal, V., & Srivastava, R. K. (2021). Pearl millet: A climate-resilient nutricereal for mitigating hidden hunger and provide nutritional security. Frontiers in Plant Science, 12, 659938. https://doi.org/10.3389/fpls.2021.659938
Yadav, D. N., Anand, T., Singh, A. K., & Wadhwa, M. (2012). Utilization of pearl millet flour for biscuit making. Journal of Food Science and Technology, 49(6), 767–773. https://doi.org/10.1007/s13197-011-0549-z
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Copyright (c) 2026 Priya, Rakesh Kumar Verma, Garima Choudhary, Sunidhi Mishra
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